Sigma Aldrich
Cell Migration & Invasion Assay
ECM512
Our lab is interested in studying the role of inflammatory kinases as a therapeutic target. We perform ELISA and western blot assays to determine the role and pro-inflammatory status of macrophages in response to pharmacological inhibition of these kinases. This kit was not the best for migration assays, the positive controls were weak and the assay is not sensitive at all. It ended up only picking up gross changes, and was difficult and expensive to trouble shoot.
Migration Assay
N/A
Bone Marrow derived macrophages were plated .5*10^6 in each chamber in FBS free media and allowed to migrate towards 10% FBS media in the lower wells under treated conditions. Cells were allowed to migrate for 12 hours prior to analysis.
Try multiple time courses and chemoattractants.
Finally got this to work after multiple attempts and trying how many cells to seed and length of migration.
Easy to seed cells in
Not user easy, bottom well doesn't align well and splashes everywhere
Not the best but pretty much the only kit out there for this type of assay.