Unclear Anti-CPT1a Antibody for Western Blot

Oncology
Johns Hopkins School of Medicine
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-CPT1A antibody

Catalog Number:

ab128568

Browse Similar Products: CPT1A Western Blot Antibodies

Our lab is interested in the expression of cpt1a in T cells and tumor cells in terms of metabolism so we used this antibody to look at the protein expression.

Experimental Design and Results Summary

Applications

Western Blot

Sample

CD8 T-cells

Primary Incubation

4% BSA in 4C overnight

Blocking Agent

5% nonfat milk

Secondary Incubation

1:5000 Mouse in 5% nonfat milk

Tertiary Incubation

N/A

Detection

ECL

Results Summary

The antibody was extremely unclear and no specific bands were shown. I did everything the protocol recommended and it still did not show anything.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

It was straightforward to use with the protocol.

The Bad

Expensive and did not show clear bands in western blot

The Bottom Line

Not sure if this is the best antibody for CPT1a but we bought it to try and it was not clean.

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