Detects Tff1 To A Lower Extent Than Some Other Antibodies, But Works

Molecular Biology
Princeton
Graduate Student

Overall

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Company:

Abcam

Product Name:

Anti-Estrogen Inducible Protein pS2 antibody

Catalog Number:

ab92377

Detects Tff1 ectopically expressed in MCF7 cells at the expected molecular weight with no non-specific bands.

Experimental Design and Results Summary

Applications

Western Blot

Sample

MCF7 ectopic expression

Primary Incubation

1:1000

Blocking Agent

5% milk

Secondary Incubation

IRDye 800CM

Tertiary Incubation

none

Detection

Licor Odyssey

Results Summary

Results showed a single band at the expected molecular weight with one large molecular weight non-specific band. Signal to noise ratio was alright, but the epitomics antibody is slightly better.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Working to identify ectopic Tff1

The Bad

Doesn't detect basal levels of TFF1

The Bottom Line

Works, but not great

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