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Anti-human CD25 FITC
356106
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Human peripheral B cells were stimulated with TLR7 agonist and activation was determined by flow cytometry after 48 hr. The antibody is easy to use and has a dynamic signal.
Flow Cytometry
Human PBMC
5 µL/test, RT, 15 min
Housemade blocking buffer containing normal sera
N/A
LSR Fortessa
Primary human PBMC were stimulated with TLR7 agonist for 48 hr and B cell activation was determined by CD25 staining. The antibody does not give the brightest signal (due to FITC being the fluorochrome), but still a very dynamic signal. (Blue histogram=unstim, Orange histogram=TLR7 agonist.)
Dynamic signal, easy to use
Not as bright as other colors
Very good antibody to determine B cell activation, and easy to use