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Anti-human CD83 Alexa Fluor 647
305316
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Human peripheral dentritic cells were stimulated with pIC overnight and activation was measured by CD83 staining. The antibody gives a bright, dynamic signal.
Flow Cytometry
Human PBMC
1 µL/test, RT, 15 min
Housemade blocking buffer containing normal sera
N/A
LSR Fortessa
Human PBMC were stimulated with pIC overnight and activation on dendritic cells (CD11c+MHC-II+) was measured using anti-CD83 AF6471. Blue histogram: unstimulated; orange histogram: pIC. The antibody is bright enough to be used at a 5x lower test size than recommended (1 µL instead of 5 µL) and results in a dynamic signal.
Very bright, dynamic signal
None
Excellent antibody to measure DC activation, bright enough to be titrated down from original test size