BD Biosciences
Anti-human CD86 Alexa Fluor 700
561124
Browse Similar Products: CD86 Flow Cytometry Antibodies
Human peripheral dendritic cells were stimulated with pIC and activation was measured by CD86 staining. The antibody gives a good, dynamic signal.
Flow Cytometry
Human PBMC
1 µL/test, RT, 15 min
Housemade blocking buffer containing normal sera
N/A
LSR Fortessa
Human PBMC were stimulated with pIC overnight and activation on dendritic cells (CD11c+MHC-II+) was measured using anti-CD86 AF700. Blue histogram: unstimulated; orange histogram: pIC. The antibody is bright enough to be used at a 5x lower test size than recommended (1µL instead of 5 µL) and results in a dynamic signal.
Dynamic signal
Not as bright as some other colors (e.g., BV conjugates)
Very good antibody to measure dendritic cell activation, delivering a dynamic signal