Anti-CKI Gamma 3 Antibody for Western Blot

January 02, 2019

LMP
University of Toronto
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-CKI gamma 3 antibody

Catalog Number:

ab37950

CKI gamma 3 has been reported to function as a serine/threonine protein kinase that phosphorylates cellular targets. Here we assess the expression of CSNK1G3 in the PC3 prostate cancer cell line. The antibody works as stated.

Experimental Design and Results Summary

Applications

Western Blot

Sample

PC3 whole-cell lysate

Primary Incubation

1:1000, Overnight at 4°C

Blocking Agent

5% skimmed milk - 30 min room temperature

Secondary Incubation

1:10000, anti-rabbit HRP conjugated antibody, 30 min at room temperature

Tertiary Incubation

N/A

Detection

ECL - film.

Results Summary

Antibody worked alright and was able to detect the protein of interest when comparing knockdown and control cell lysates. Size ~ 50kDa.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Antibody works alright

The Bad

Had to load quite a bit of total protein to see band

The Bottom Line

Works as stated.

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