Cell Signaling Technology
Rictor monoclonal Antibody
9476
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Our lab studies the role of mTOR signaling in macrophage polarization and function. We perturb key signaling molecules within this pathway to study this pathway. We deleted Rictor protein in macrophages to study the role of mTORC2 in macrophage polarization and function. The antibody is easy to use and has great specificity.
Western Blot
Murine macrophages (WT and Rictor KO)
1:2000 in 4% BSA/TBST
5% Dry milk/TBST
1:5000 anti rabbit HRP
None
Invitrogen ECL Pico
We are able to detect Rictor protein in our samples and are able to identify the appropriate band by analyzing it in Rictor KO macrophages since we do observe protein in these cells.
28877476
N/A
Easy to use and great specificity
Great monoclonal antibody to detect Rictor protein via immunoblotting.