Abcam
DCFDA / H2DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit
ab113851
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This kit is not much of a kit. It contains the DCFDA reagent, already reconstituted, an oxidative stress inducer, and a diluting buffer. Given that you will put the reagent into cell culture, you don't need the dilution buffer. Also, the carboxy-methyl-DCFDA reagent can be purchased for a better price at other suppliers and is retained in the cell for longer. Finally, you can use peroxide or multiple other positive controls. Overall, this kit will work for very strong inducers, but testing in standard breast cancer cell lines with effective doses of chemotherapy showed no signal above the variable background. I recommend purchasing cm-DCFDA or dihydroethidium.
Flow cytometry
Human breast cancer cells
Product is added to cell culture at 20 µM for 4–24 hr. Appropriate positive and negative controls such as n-acetylcysteine and peroxide are used. Cells are washed, trypsinized, and measured by flow in the FITC channel.
Order dry carboxy-methyl DCFDA from other suppliers for cheaper and more robust results.
Showed positive signal for strong oxidative conditions, but weak signal overall.
N/A
Very easy to use
Low sensitivity
Try other reporters such as cm-DCFDA first. They are cheaper and more robust.