Great Nestin Antibody from GeneTex for Immunocytochemistry

Department of Laboratory Medicine
MacKay Memorial Hospital
Research Assistant

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

GeneTex

Product Name:

Nestin antibody

Catalog Number:

GTX133111

This antibody was used to detect Nestin expression in neural stem cells by immunocytochemistry analysis.

Experimental Design and Results Summary

Applications

Immunocytochemistry

Sample

Neural stem cells

Primary Incubation

1:100 dilution overnight at 4°C in blocking reagent

Blocking Agent

5% normal goat serum in 0.1% tween 20 / PBS

Secondary Incubation

1:200 dilution 1 hr at 25°C in blocking reagent (Alexa Fluor 594 dye)

Tertiary Incubation

N/A

Detection

Fluorescence microscope

Results Summary

This antibody perfectly detected Nestin expression by ICC/IF in NSCs.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

High sensitivity

The Bad

Minor background signals

The Bottom Line

Antibody has high sensitivity and perfectly detected Nestin expression by ICC. We will continue to use this antibody

Share your experience with other scientists. Write a Review! »

Join the discussion