Cell Signaling Technology
Phospho-Akt (Ser473) Antibody
9271
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Our lab is interested in immunology, specifically metabolism of T cells. Therefore, looking at pAKT has always been a site we use to look at mTORC2 and the PI3K pathway. This antibody helps us look at the effects of glutamine metabolism for T cells in normal glutamine concentration conditions, versus low glutamine conditions (nutrient deprivation-like).
Western Blot
Murine T cells
1:5000 in 4°C overnight
5% nonfat dry milk
1:5000 anti-rabbit HRP-linked
N/A
ECL Pico
The bands always show up bright and strong at the correct kDa. There isn't any nonspecific binding, so it is very reliable every time we use the antibody.
Bright band and correct MW on western blot.
None whatsoever
It is worth the price, and our lab uses a smaller dilution than the recommended dilution by CST and it still works great.