Bright Band in Western Blots for pS6

Oncology
Johns Hopkins School of Medicine
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cells Signaling Technology

Product Name:

Phospho-S6 Ribosomal Protein (Ser235/236)

Catalog Number:

2211

Our lab has used this antibody to look at the Phospho-S6 in murine T cells. We were interested in how the mTOR can be regulated through glutamine metabolism so this was one antibody we used for western blot. It is one of the best on the market for staining pS6.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Murine T cells

Primary Incubation

1:10,000 in 4°C overnight

Blocking Agent

5% nonfat dry milk

Secondary Incubation

1:5000 anti-rabbit HRP-linked

Tertiary Incubation

N/A

Detection

ECL Pico

Results Summary

The signal is always very bright and clean. We were able to see obvious differences in glutamine concentration conditions.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright band and doesn't need that high of a concentration to see signal

The Bad

None

The Bottom Line

Definitely a must-buy for western blots with pS6. It is one of the best ones we have used on the market compared to other brands.

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