A Great Turnkey Solution for STED Microscopy

October 11, 2018

Georgetown University Medical Center
Pharmacology and Physiology
PhD Candidate

Overall

Performance

Ease-of-Optimization

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Company:

Abberior instruments

Product Name:

STEDYcon

Catalog Number:

Stedycon

Abberior instruments produces a nifty modular system that enables ANY modern microscope to gain confocal and STED microscopy capabilities.In literally minutes, we were able to upgrade our existing confocal to a state of the art super resolution nanoscope.

Experimental Design and Results Summary

Application

STED microscopy on anti-CX3CR1 IF prep

Protocol Overview

Standard IF prep, with abberior 635P-conjugated secondary Ab. Mounted in ProLong AF mounting medium.

Tips

Make sure you use compatible fluorochromes!

Features Summary

4 laser lines for confocal, 2 for STED

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Super easy to set up and use!

The Bad

The STED principle doesn't work with all dyes and FPs.

The Bottom Line

Super easy and reliable way to go beyond the capacities of any microscope package.I find it crazy that we can implement Nobel-prize winning super resolution imaging technology in our core just a few short years after it's discovery!

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