Easy to Use Automated Magnetic Cell Sorting

August 21, 2018

BlueskyReddit
Memorial University of Newfoundland
Division of BioMedical Sciences, Faculty of Medicine
Research Assistant

Overall

Performance

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

MIltenyi Biotec

Product Name:

autoMACS Pro Separator

Catalog Number:

130-092-545

Info:Supplier Page View Product Specs View Product

This review is for the autoMACS Pro, which has been discontinued and replaced by the autoMACS Neo.

The autoMACS Pro Separator from Miltenyi Biotec allows full automation of magnetic cell sorting of your desired cell population of interest for your research. It has the capability to isolate cells from your purified heterogeneous cell sample, but also directly from primary tissue sources such as whole blood or bone marrow. If you are already experienced with MACS products for manual cell separation, the transition to automated cell sorting is straight forward as several of the reagents used by both techniques are the same. MACS Microbead technology in combination with the autoMACS Pro Separator offers an alternative or complement to flow cytometry based sorting, and is compatible with several downstream applications including but not limited to cell culture, functional assays, flow cytometry, PCR, and large scale gene expression analysis. Our lab routinely used the autoMACS separator to obtain highly pure immune subsets from peripheral blood mononuclear cells.

Experimental Design and Results Summary

Tips

It is important to have a single cell population of cells to avoid clogging and adequate separation. There are different programs that are designed to prioritize cell recovery or purity. Thus some preliminary experiments may be required to evaluate which automated separation protocol provided by the instrument is suitable for your experiments in order to achieve your desired balance of cell recovery and purity. Separated cell populations should be routinely checked by flow cytometry to ensure the purity of the isolated cells.

DOI or PMID #

26307036

Image Gallery

Summary

The Good

User friendly interface. Easy to use and maintain. Full automation is possible, or a combination of manual and automation is also possible. Pre-set programs that allow you to customize your separation to suit your needs. Reagents come with detailed protocols and are already optimized for use with the instrument. Ability to add attachments as user needs grow.

The Bad

Reagents and buffers can get expensive depending on frequency of use.

The Bottom Line

If your lab frequently performs cell separations to obtain pure populations of cell from heterogeneous samples, then the autoMACS Pro Separator should be seriously considered. Compared to flow cytometry-based sorting applications, sample preparation is less complicated and time consuming and less technical expertise is required.

Join the discussion