F4/80 in Immunofluorescence Imaging

Our laboratory studies T Cell Acute Lymphoblastic Leukemia (T-ALL), a cancer of developing T cells. In order to examine the composition and organization of the tumor microenvironment, our lab utilizes a wide variety of markers to identify specific cellular subsets of the tumor microenvironment. We picked this antibody for immunofluorescence imaging knowing that it would characterize the architecture of the tumor microenvironment in T-ALL in our mouse model. F4/80 is a protein that is primarily expressed on macrophages, but also certain subsets of monocytes and dendritic cells. Using a direct conjugate for IF imaging is not necessarily recommended, but I was able to get it to work.