Use Of Isotype Rat IgG1 Kappa Isotype Control in Flow Analysis

February 22, 2018

Pharmacology and Experimental Neuroscience
University of Nebraska Medical Center
Graduate Research Assistant

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Company:

Invitrogen Antibodies

Product Name:

Rat IgG1 kappa Isotype Control, PerCP-eFluor 710

Catalog Number:

46-4301-80

In this experiment control cells were treated with permeabilization buffer and labeled with isotype antibody in order to distinguish background fluorescence from primary antibody signal.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

CD4+ T cells from mouse splenocytes

Primary Incubation

0.1ug/1.0x105 cells for 1 hour

Blocking Agent

Gamma globulin

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD LSRII Flow Cytometer

Results Summary

This antibody isotype reveals high amounts of background fluorescence in flow analysis.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

This antibody can control for background fluorescence

The Bad

This antibody demonstrates very high levels of signal to noise ratio

The Bottom Line

This isotype antibody must be used with the primary PerCp-eFluor 710 antibody to control for the high amounts of background noise.

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