Using The IFN Gamma Monoclonal Antibody (XMG1.2), FITC To Check The Purity Of A Naive T Cell Polarization To The Th17 Phenotype

December 22, 2017

Pharmacology and Experimental Neuroscience
University of Nebraska Medical Center
Graduate Research Assistant

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Quality of Results

Ease-of-Optimization

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Company:

Invitrogen Antibodies

Product Name:

IFN gamma Monoclonal Antibody (XMG1.2), FITC

Catalog Number:

11-7311-82

For this experiment Th17 cells were polarized from naive CD4+ T cells that were negatively selected for CD4 using an R&D column and a single cell suspension of splenic and lymph node cells. Following polarization the purity of the polarization was checked utilizing the IFN gamma Monoclonal Antibody (XMG1.2), FITC.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse CD4+ T cells

Primary Incubation

0.1ug/1.0x105 cells for 1 hour at 4 degrees celcius

Blocking Agent

Gamma globulin

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD LSRII Flow Cytometer

Results Summary

This antibody was easily titered and showed a completely pure population of Th17 cells with no IFN gamma being produced.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

The antibody was easily titered

The Bad

N/A

The Bottom Line

This antibody works well to detect intracellular cytokines that may be present at very low levels inside the cell.

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