Controlling For Background Signal In Flow Cytometry Utilizing Alexa Fluor® 647 Rat IgG1, kappa Isotype Ctrl Antibody

January 04, 2018

Pharmacology and Experimental Neuroscience
University of Nebraska Medical Center
Graduate Research Assistant

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Company:

Biolegend

Product Name:

Alexa Fluor® 647 Rat IgG1, κ Isotype Ctrl Antibody

Catalog Number:

400418

CD4+ T cells were isolated from mice and polarized to a specific phenotype using cytokines and antibodies. After polarization the phenotype of the cells was checked utilizing flow cytometry and the Alexa Fluor® 647 Rat IgG1, κ Isotype Ctrl Antibody in order to control for the signal to noise ratio to differentiate non-specific background signal from specific antibody sign.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

CD4+ T cells from mice

Primary Incubation

0.1ug/1.0x105 cells for 1 hour

Blocking Agent

Gamma globulin

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD LSRII Flow Cytometer

Results Summary

This control is very effective in differentiating non-specific background signal from specific antibody sign during flow cytometry.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

This control is very inexpensive

The Bad

N/A

The Bottom Line

I highly recommend this control as it gives great results and it us very inexpensive.

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