TCR pan gamma delta PE Cy7 Antibody

Institute of Health and Biomedical Innovation
Queensland University of Technology
PhD Student

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Company:

Beckman Coulter

Product Name:

TCR pan gamma delta PE Cy7

Catalog Number:

B10247

Browse Similar Products: TCR Flow Cytometry Antibodies

To test if Beckman's anti-human TCR gd PECy7 works, I stained human PBMC with this antibody and did a serial titration. This trial experiment suggests that this antibody can be used to identify total gamma delta T cells in human PBMCs.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

2e6 Human PBMC in FACS buffer

Primary Incubation

30min at RT in the dark

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

BD Fortessa 4

Results Summary

gdTCR positive cells were identified from the titration experiment.

DOI or PMID #

NA

Additional Notes

I titrated the antibody by performing seven 2-fold dilution starting at 1:20.

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Summary

The Good

Antibody worked

The Bad

NA

The Bottom Line

This antibody can be used to identify gamma delta T cells in PBMC.

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