Reliable Temperature Centrifuge

University of California, Merced
Molecular and Cell Biology
Graduate Student

Overall

Performance

Ease-of-Optimization

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Company:

Beckman Coulter

Product Name:

Allegra X-14 Constant Temperature Centrifuge from Beckman Coulter

Catalog Number:

A99464

Our research goal is to define the cellular contributors to systemic autoimmune disease. This centrifuge has never let us down. It can be used for a variety of experimental set-ups from 2 degrees to room temperature. The buckets are easily changed with inserts to support a variety of plastics including cell culture plates 50mL conical tubes or 15mL conical tubes. It rarely needs maintenance or upkeep and will last you forever.

Experimental Design and Results Summary

Application

Cell Culture/ Immunology

Starting Material

50mL conical tubes or 15mL conical tubes

Protocol Overview

Set the brake (deceleration) and acceleration according to indivudual needs . Set RPM/RCF, temperature and time. Set individualized program options by selecting the corresponding button on the display and entering a value within the range shown at the top of the screen then select enter. Once specifications are set be sure to click the enter button again then press start. (Start button alone is not sufficient to start your spin).

Tips

You can change any of the settings even after the spin has started.

Results Summary

If you set your parameters correctly the centrifuge displays the temperature, RPM/RCF and remaining spin time on the top row of the display. Your cells or other items will pellet nicely. If there is an imbalance the centrifuge will automatically stop after a moment. Although damage can still be done so it is best to press stop instead of waiting for the automatic stop to ensure product life.

Features Summary

Interchangable buckets to support a wide range of products.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

It realiable works with little to no maintenance.

The Bad

No heating elements.

The Bottom Line

Reliable centrifuge that will work as long as you do anywhere from 2 degrees Celsius to room temperature.

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