Competitive CD11b-APC Conjugated Antibody

August 23, 2017

Biomedical Sciences
Cornell University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Tonbo Biosciences

Product Name:

CD11b APC

Catalog Number:

20-0118 (T025)

Our lab studies all-trans retinoic acid-induced differentiation of HL-60 cells; CD11b is a surface marker we routinely characterize over the course of 72 h treatment periods. Its expression is upregulated, going up to about 60-80% positive (from a background gate of 5% positive in untreated controls) over 72 h. We used this product to characterize these changes in expression.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Live HL-60 cells

Primary Incubation

2.5 ul antibody in 200 ul PBS for 1 h at 37 degrees C

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry - 488 nm laser line through 505 longpass mirror and 530/30 filter

Results Summary

After treating HL-60 cells with retinoic acid for 72 h, we were able to detect significant upregulation in CD11b expression by flow cytometry using this antibody. Our results were consistent with previous results in which we used a different CD11b antibody, though background fluorescence in untreated controls was higher.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Pricing is very competitive, little protocol optimization was required

The Bad

This product seemed to have high background signal compared to other CD11b antibodies we have used.

The Bottom Line

Very competitively priced CD11b antibody. This seems like it may have higher background than others, but we were still able to get results consistent with other CD11b antibodies we have used (such as those from BD) by percent change in expression without optimization of our staining protocol. It is possible that background could be minimized with further optimization if looking for small changes in protein expression.

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