Molecular Probes (Thermo Fisher Scientific)
Isolectin GS-IB4 Alexa Fluor 647 conjugate
I32450
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Marking specific cell types in tissue section has always been a challenge. Isolectin-AF647 conjugate comes handy to mark endothelial cells and vasculatures in tissue sections. In addition to ECs, it can bind to other cell types like microglial cells and neuronal cells.
Immunofluorescence
Fixed mouse tissue sections
Block rehydrated FFPE tissue sections with 3% BSA in PBS for 30 min and then incubate with Isolectin-AF647 (1:200-1:500) for 2 hours at RT. Wash three times with 3% BSA and counter stain with Hoechst 33342 dye. We have included Isolectin-AF647 with primary and secondary antibodies when we stain the section with antibodies. Always works. Did not encounter nonspecific staining.
Do not thaw and refreeze. It will lead to loss of binding.
Mouse heart section was incubated with 1:200 diluted isolectin-AF647 (crimson color) for 2 hours at RT and then washed 3X with PBS. The section was simultaneously stained with alpha-actinin (green) and Hoechst 33342 dye (blue).
N/A
No background staining and works all the time
In addition to endothelial cells, it binds to neuronal and immune cells.
Highly reliable reagent to mark endothelial cells in heart and retina.