Atlas Antibodies
Anti-ODF2 antibody produced in rabbit
HPA001874
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Mature centrioles have assessor structures (called distal and subdistal appendages) attached near their distal ends. My work attempts to elucidate the function of the subdistal appendages of the centriole. We double checked the localization of ODF2 to confirm that it localizes to the subdistal appendages. We also checked the localization of other proteins. We also needed this reliable antibody to confirm depletion of the proteins with RNAi and CRISPR. The antibodies also allowed us to determine which conditions lead to the mislocalization of these proteins (Cep128, Centriolin, ODF2, Kif2a, cep170, ninein ……).
Immunofluorescence
RPE1 cells, HeLa cell and IMCD3 cells, fixed with Methanol (-20C)
1:500 dilution of antibody in PBS with 3% BSA and 0.1% triton
PBS with 3% BSA and 0.1% triton
Anti rabbit Alexa 594 (from molecular probes)
DAPI
Fluorescent microscopy
This ODF2 antibody gives a clear staining of ODF2 at the subdistal appendages of the centrioles.
27818179
N/A
Antibody works in human and mouse cells and is stable for over 1 year at 4C
Nothing bad
Its a very good antibody to detect ODF2. We use it for immunofluorescence but it also works for western blotting.