Phosphorylation Of STAT6 In Mouse B Cells

June 02, 2017

Immunology
Northwestern University
Grad Student

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Company:

eBioscience™ from Invitrogen Antibodies

Product Name:

Phospho-STAT6 (Tyr641)

Catalog Number:

12-9013-42

Goal of the research was to determine phosphorylation of STAT6 after stimulation with IL-4 in mouse B cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenic B cells

Primary Incubation

5ul (one test), 4 degrees, 1 hour

Blocking Agent

Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

None

Results Summary

Clear staining of phosphoSTAT6 after stimulation.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy to use, no troubleshooting required, comes ready to use

The Bad

None

The Bottom Line

Great antibody to measure phosphoSTAT6 by flow

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