Rhodamine LCA For Staining Chick Embryo Vasculature

The Scripps Research Institute
The Cell and Molecular Biology
Assistant Professor

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Vector Laboratories

Product Name:

Rhodamine Lens Culinaris Agglutinin

Catalog Number:

FL-4042

The Lens Culinaris Agglutinin is the one of the few lectins, which efficiently bind to the vascular endothelium of the chick embryo. Immunofluorescent staining of chick embryo vasculature is important in developmental biology research and in cancer related studies involving the use of human tumor cells or tumor cells of any other species since chick embryo is a naturally immunodeficient host. The Rhodamine Lens Culinaris Agglutinin is especially helpful to illuminate chick embryo vasculature when tumor cells are tagged with a "green" immunofluorescent protein (e.g., GFP).

Experimental Design and Results Summary

Application

Immunofluorescent staining of the vasculature in the chick embryo

Starting Material

Chick embryos incubated for 12-15 days.

Protocol Overview

Chick embryo (day 10-15) is inoculated with 0.1-0.2 ml of Rhodamine LCA diluted 1:20-1:50 in PBS.

Tips

The chorioallanoic membrane (CAM) vasculature could be visualized in fluorescent microscope immediately without any fixation or could be fixed in Zn-formalin for analysis later.

Results Summary

Rhodamine LCA produces excellent staining of chick embryo vasculature.

DOI or PMID #

N/A

Additional Notes

The image depicts normal CAM vasculature in a day 13 chicken embryo.

Related Categories

Image Gallery

Summary

The Good

Excellent stable staining of the vasculature with minimal background and minimal leakage of lectin into surrounding tissue.

The Bad

None detected

The Bottom Line

Rhodamine LCA conjugate is excellent lectin to stain chick embryo vasculature. This reagent is especially useful to stain tumor-associated vasculature in GFP-tagged tumors.

Join the discussion