Anti-Mouse Ago2 Monoclonal Antibody For Western Blot

Cell Biology and Molecular Genetics
University of Maryland, College Park
Post-doctoral researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Wako Chemicals USA, Inc.

Product Name:

Anti Mouse Ago2, Monoclonal antibody

Catalog Number:

018-22021

One ongoing project going on in the lab is studying CD4+ T cells in in vivo mouse models. We study various molecular pathways which requires reliable antibodies to measure protein levels from cells and tissue.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse brain homogenate

Primary Incubation

Anti-mouse Ago2 antibody at 1:1000

Blocking Agent

5% milk 5% goat serum

Secondary Incubation

LI-COR anti-mouse IRDye secondary antibody

Tertiary Incubation

None

Detection

LI-COR Odyssey

Results Summary

The antibody stains a single band at the expected molecular weight.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Specific staining of desired protein

The Bad

None

The Bottom Line

Great antibody for Ago western blotting in mouse tissues.

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