Agilent Technologies
QuikChange II XL Site-Directed Mutagenesis Kit,
200521
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In our lab we study T cell receptors cloned from T cells isolated from patients. We clone these TCRs into lentiviral expression vectors. In the process of optimizing the sequence of these TCRs to facilitate optimal pairing of the alpha and beta chains upon transduction, we use the site directed mutagenesis kit to introduce point mutations into TCR sequence. This kit is easy to use and produces results with minimal effort and time.
Molecular cloning
Plasmid DNA
Design primers using the Agilent website tool. Follow the protocol and set up a PCR reaction to introduce the desired mutation(s) into the DNA. Use the PCR product to transform bacteria, isolate DNA from resulting colonies. Sequence the plasmid DNA to confirm the sequence desired.
Follow the protocol, very straight forward. Use the website tool.
Several positive minipreps in the first mutagenesis reaction
N/A
Fast and reliable
None
Great kit to use for introducing mutations in plasmid DNA.
Product Name: QuikChange® II XL site-directed mutagenesis kitSupplier Page