Red Blood Cells For Reverse ABO Blood Typing

March 01, 2017

BlueskyReddit
Albert Einstein College of Medicine
Clinical Research
Pre-doctoral researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Immucor

Product Name:

Reagent Red Blood Cell Kit

Catalog Number:

0002345

In the course of our clinical study, we realized that we needed to blood type the patients under analysis. As we only had plasma available (no RBCs), we looked to Immucor's reagent red blood cell kit to 'reverse blood type.' The kit was recommended to us by a clinical hematology lab, and we found it to be incredibly reliable (given the appropriate controls) and practical for our needs.

Experimental Design and Results Summary

Application

Blood typing (reverse method)

Starting Material

Human serum or plasma

Protocol Overview

Place 100 ul of plasma from each patient into an 'A' labeled tube and another 100 ul from each patient into a 'B' labeled tube. Add 1 drop of A1 RBCs to each 'A' labeled tube and 1 drop of B RBCs to each 'B' labeled tube. Spin all tubes at 3400 rpm for 20 seconds and read immediately (blinded reads by two independent readers is preferred).

Tips

Aliquot all plasma prior to adding any RBC reagent to save time. Add RBC reagents and subsequently spin in sets to avoid letting samples sit for too long following the spin before being read. Read immediately (again, two readers is preferred).

Results Summary

Type A = Reactive to Reference B cells, Type B = Reactive to Reference A1 cells, Type AB = Reactive to neither Reference cell, Type 0 = Reactive to Reference A1 & B cells

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Simple protocol, reliable method, time efficient.

The Bad

Not as accurate as forward blood typing. Plasma/serum from patients with severe disease may yield strange/altered results.

The Bottom Line

I recommend this kit for anyone who needs to conduct reverse blood typing on human plasma or serum samples. The reagents are very simple to use, the procedure in total does not take much time, and the results of the test are known immediately. My only hesitation is using the reagents on incredibly sick study populations, as various factors can affect the blood typing reagents.

Join the discussion