KPL, a SeraCare company
HistoMark® RED
55-69-00
In my studies, I needed to visualize genetically β-galactosidase tagged molecules in mouse tissue with a fluorescent marker in conjunction with other markers. This assay kit allowed me to do that using the Biotin + streptavidin-phosphatase antibody system.
Immunohistochemistry
Mouse retina tissue
Fixed retina tissue is blocked (1% Goat Serum, 0.3% Triton in PBS for 1 hour at room temperature) and then stained with 1:40 biotinylated primary antibody in blocking solution and incubate at 4°C overnight. The tissues were then stained with streptavidin-phosphatase the same was and washed with PBS. The PhThaloRed solution is mixed with the substrate solution and added to the tissue for a 20 minutes stain at room temperature.
Only prepare Histomark solution right before use.
Though complicated, this stain was the only way we have been able to successfully fluorescently visualize our target cells that are tagged with β-galactosidase.
N/A
Works well in mouse tissue
None
This assay kit works well for fluorescently tagging any phosphatase bound molecules in mouse tissue.