Use of Dystrophin (Dys1, Dy4/6D3) antibody from Leica Biosystems on skeletal muscle section

November 23, 2016

Pathology
BWH
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novocastra by Leica Biosystems

Product Name:

Mouse Monoclonal Antibody Dystrophin (Rod Domain) [Dy4/6D3]

Catalog Number:

DYS1-CE

 

Browse Similar Products: Dystrophin Immunohistochemistry Antibodies

This antibody was used to analyze the presence of Dystrophin on adult mouse skeletal muscle cryosections. This monoclonal anti-Dystrophin (Dys1, clone Dy4/6D3) antibody appears highly specific to Dystrophin. Staining with this antibody is subsarcolemmal, distributed under the muscle fiber plasma membrane.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Adult mouse skeletal muscle cryosection

Primary Incubation

1:200, overnight, at 4C in Blocking agent, on slide

Blocking Agent

TBS, 1% FBS, 0.1% TritonX100, 30min, at RT

Secondary Incubation

1:500, AlexaFluor-488-conjugated anti-mouse IgG secondary (Molecular Probes), overnight, at 4C in Blocking agent, on slide (counterstaining of nuclei with dapi in blue)

Tertiary Incubation

N/A

Detection

Fluorescent microscope, FITC/GFP filter for AlexaFluor-488 detection

Results Summary

This antibody works well on fixed tissue section of mouse skeletal muscle. High specificity for the cell membrane (sarcolemma), marks the domain just under the plasma membrane.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Good specific signal at cell membrane by immunofluorescence

The Bad

Signal is moderately strong

The Bottom Line

Good antibody overall. Highly recommended for immunofluorescence study of mouse skeletal muscle on tissue sections. This antibody reacts with Dystrophin and marks essentially the intracytoplasmic domain of the muscle fiber's sarcolemma.

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