Fluidigm
Anti-Human CD25-169Tm
3169003B
Browse Similar Products: CD25 Flow Cytometry Antibodies
Our group studies T cells in the blood of untreated chronic HIV+ individuals. We are constantly expanding and optimizing our T cell panels to measure various T cell subsets, activation, and inhibitory receptor phenotype. We recently have begun optimizing mass cytometry panels to expand our panels significantly. We chose CD25-169Tm because it was commercially available.
Flow Cytometry
Human PBMC
0.25uL CD25-169Tm in 100uL PBS with 10^6 cells; 25 minutes; room temperature
None.
CyTOF (Fluidigm)
The attached image illustrates the importance of having fluorescence cytometry controls to compare to when titrating antibodies for mass cytometry. Antibodies should be titrated individually for optimal stain index by fluorescence cytometry. Then, cells from the same individual should then be stained in tandem with fluorescently-conjugated CD25 and various volumes of CD25-169 to determine which volume of CD25-169 to pick. As illustrated in the image, increasing volumes of CD25-169 can quickly increase false positive signal that is less obvious than it is in fluorescence cytometry. At 0.25uL, CD25-169Tm works great to detect CD25+ cells from human PBMC samples after overnight SEB stimulation (1ug/mL).
0.25uL/sample of CD25-169Tm works great!
Nothing wrong with product; just be sure to titrate properly!
CD25-169 works great and is a nice option for incorporating CD25 into a mass cytometry panel.