Antibodies Incorporated
Anti-Matrix Metalloproteinase 9
75-100
Browse Similar Products: MMP9 Western Blot Antibodies
This antibody was used in a study investigating the effects of N-glycosylation within Matrix Metalloproteinase-9 (MMP9) by characterizing the intracellular organelle trafficking of the N-glycoyslation mutants of MMP9. It has been published in Traffic in 2015 (Duellman et al., Functional Roles of N-Linked Glycosylation of Human Matrix Metalloproteinase 9, Traffic. 10, 1108-26). Anti-MMP9 was used to detect secreted MMP9 from rat macrophage cells (RAW264.7). Conditioned medium was stained for MMP9 to quantify the levels of secreted MMP9 in PMA stimulated cells using different secretion or N-glycosylation inhibitors.
Western Blot
Conditioned media from PMA stimulated rat RAW264.7 cells in the absence or presence of different secretion or N-glycosylation inhibitors.
1:1000 in 2% milk incubated at 4C overnight
5% milk
Anti-Mouse-HRP (1:5000) in 2% milk incubated for 1 hour at room temperature.
N/A
SuperSignal West Femto ECL Substrate (Thermo #34094)
Anti-MMP9 worked great in numerous applications and with different sample preparations including endogenous or exogenous protein detection.
Reactivity against human, rat, and mouse.
This antibody has worked great for western blotting purposes and is sold at an extremely reasonable price compared to other commercial vendors.
None to report.
I would strongly recommend this antibody to other investigators studying MMP9.