IL-1b ELISA

August 26, 2016

Jecure Therapeutics
Jecure Therapeutics
Scientist II

Overall

Quality of Results

Ease-of-Optimization

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Company:

Boster

Product Name:

Human IL-1 Beta PicoKine™ ELISA Kit

Catalog Number:

EK0392

We are a pharmaceutical startup optimizing cell based drug assays where one of our endpoints was observing the differences in mature IL-1b production in the media. I have compared several kits from the most expensive versions of R&D, over eBioscience/Affymetrix to a more budget Boster's kit. The aim was to decide what is the best price for the value and quality we are getting for reliable and replicable ELISA analyses.

Experimental Design and Results Summary

Application

ELISA

Starting Material

protein concentrations in cell culture media

Protocol Overview

Followed it very precisely from the BOSTER's manual. Used PBS as a wash buffer (not included in the kit).

Tips

Carefully follow all the steps and incubation times from the protocol; must use a multichannel pipette

Results Summary

This assay was used to test the effect of increasing concentrations of a drug on IL-1b levels (dose response). The kit reported similar data obtained from the other two kits (R&D and eBiosciences), with a slightly larger standard deviations.

Additional Notes

None

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Summary

The Good

Results replicated the findings of the previous kits and the assay had good sensitivity/ instructions on how to perform assay and analyze data

The Bad

Various multi step procedures decrease inter-assay reproducibility and take longer to perform as compared to R&D & eBiosciences instant kits. The CV values of the lower standard curve points were 10-20%, which was also a bit higher than the two aforementioned kits.

The Bottom Line

I would use this assay in an academic lab for a project where no more than 2-3 96-well plates will be used. It should be done carefully by a scientist that has previously performed ELISA assays. There are many steps to it. It is a good quality budget option to the other leading kits on the market. I would not recommend it for a larger drug screen assay where more than 5 ELISAs kits ought to be used.

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