H3K4me3 Antibody Abcam Review

August 12, 2016

Genetics
Yale
Tech

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade

Catalog Number:

ab8580

We study epigenetics in mouse ES cells, looking at how knocking out some proteins affects histone or DNA modifications.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse ES cells

Primary Incubation

1:1000 4C overnight

Blocking Agent

5% milk

Secondary Incubation

1:3000 in 5% milk for one hour at RT

Tertiary Incubation

N/A

Detection

Exposure with SuperSignal™ West Femto Maximum Sensitivity Substrate, imaged with apparatus from Bio-Rad

Results Summary

Great antibody, very clear, strong signal, chIP-grade

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Strong clear band, strong antibody

The Bad

Really expensive

The Bottom Line

Would buy again, great antibody, though a bit expensive.

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