Boster
Anti-CD44 Picoband Antibody (against N-terminus)
PB9333
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Browse Similar Products: CD44 Western Blot Antibodies
Polyclonal rabbit antibody from Boster against N-terminal domain of CD44, PB9333, was evaluated in western blotting with the lysates of human cancer cells positive for expression of CD44 and recombinant N-terminal domain of CD44. The antibody produced very good results consistent with expected mol. wt. of analyzed proteins.
Western Blot
Tumor cell lysate and recombinant protein
Overnight, at 4-8 degrees Celsius, at 1 ug/mL in 5% milk/PBS/0.05% Tween 20
5% milk in PBS/0.05% Tween-20 (5% milk/PBS/Tw)
Goat anti-rabbit antibody conjugated with HRP at 1:5,000 in 5% milk/PBS/Tw
None
SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific)
The rabbit antibody PB9333 from Boster produced very good results in western blotting with the lysate of human CD44-positive cancer cells as well as with the recombinant N-terminal protein. The detection was specific, resulting only in the protein bands of expected mol. weights, i.e., 80 kDa full-length CD44, and approximately 50 kDa CD44 cleavage product in the cell lysate, and approximately 50 kDa band with the recombinant N-terminal protein of 43-48 kDa.
The antibody is also capable of detecting shed CD44 in conditioned medium
High specificity and stability
None detected
I would highly recommend this antibody for detection of CD44 in human cells by western blotting.