Bethyl
anti-G6PD antibody
A300-404A
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We study Glucose 6-Phosphate Dehydrogenase in our lab. There are several reasonably good antibodies on the market but we think that the Bethyl one is the best in general. We have been using this antibody for several years and are very happy with it.
Western Blot
Tib73 cytosolic extract
1:1000, overnight, 4 deg C, rocking
Odyssey Blocking Buffer
1:20,000, 1 hr, room temp
None
LiCor Odyssey Scan
We obtain very clean bands on Western Blot with this antibody. Note, we have found that the bands sometimes are hard to detect unless we use the right kind of Odyssey Blocking Buffer and a good strong fluorescent infrared secondary antibody (in this case, we used LiCor antibody 925-68073). For this particular experimental setup, we don't like to use Casein Buffer. We prefer Licor 927-40100. That is the way to get the brightest bands.
Excellent antibody!!!
No drawbacks!
We recommend this antibody!