Santa Cruz Biotechnology
Caspase-1 p10 Antibody (M-20)
sc-514
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This rabbit anti-mouse caspase-1 antibody can recognize protein extract from various mouse tissues and mouse cell lines. Caspase-1 has a 45kD pro-enzyme and two functional cleavaged products, this antibody can recognize both which is great. The dilution factor is about 1:2000, if the protein prep is clean. However, there is background protein shown on the blots too (I used a homogenized mouse organ), if the protein is a mixed sample. It also works with immunofluorescent microscopy. It is a relatively okay price compare to most primary antibodies.
Western Blot
Homogenized mouse organ
1:2000, 5% milk TBST
5% milk TBST
1:2000 TBST
NA
ECL
As shown in the fig, the major caspase-1 proenzyme is clean and strongly recognized. However, much background still existed with the band. This antibody can also recognize cleaved caspase-1 (not shown in the fig) and can also work with IFA.
None
Easy to operate, detects pro and cleaved enzyme
Background
Can work in both western and IFA