Total RNA Extraction from Whole Blood

March 22, 2016

Boston University
Center for Regenerative Medicine
Postdoctoral Associate

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Company:

PreAnalytix

Product Name:

PAXgene Blood RNA Kit

Catalog Number:

762164

Total RNA can be extracted from whole blood using the PAXgene Blood RNA kit. Whole blood is collected from an individual into a PAXgene Blood RNA tube. The kit is phenol/chloroform free. It is a silica membrane based protocol.

Experimental Design and Results Summary

Application

Total RNA for PCR

Starting Material

Whole blood

Protocol Overview

Initially, cells are pelleted, washed multiple times and then protein is degraded. Samples are homogenized using the reagent provided in the kit, washed and DNAse treated. Then washed again multiple times and finally eluted using the buffer provided in the kit. The concentration and purity are determined using the Nanodrop or bioanalyzer.

Tips

Use a syringe with needle to homogenise the samples.

Results Summary

Good total RNA yield is derived from the extraction process but the purity is generally low. Good kit in the absence of alternative methods.

Additional Notes

None

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Summary

The Good

Easy to use kit. Allows total RNA to be extracted from whole blood.

The Bad

Expensive, time consuming and low purity.

The Bottom Line

Consider alternative methods of total RNA extraction before opting for this kit. The total RNA extracted could be from every cell-type or extracellular vesicles present in whole blood.

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