Decent a-Tubulin Antibody

March 01, 2016

Research
University of North Carolina at Chapel Hill
Laboratory Technician

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Sigma Aldrich

Product Name:

Monoclonal Anti-α-Tubulin antibody produced in mouse

Catalog Number:

T5168

Our lab performs a ton of western blots and we recently needed to blot for alpha-tubulin. We stumbled upon this antibody after borrowing an aliquot from another lab, and have since purchased it for ourselves.

Experimental Design and Results Summary

Applications

Western Blot

Sample

786-0, RPTEC, and RCC4 Cell Lysates

Primary Incubation

1:1000 Dilution Overnight in 5% BSA in 1X TBS-T at 4C

Blocking Agent

5% Milk in 1X TBS-T

Secondary Incubation

1:5000 dilution of HRP-conjugated Mouse secondary in 5% Milk in TBS-T for 1 hour at RT

Tertiary Incubation

None

Detection

ECL --> Autoradiography Film via HRP-Conjugate Secondary Antibody

Results Summary

We get very clean results with this antibody. It comes up well on film, works with low protein levels, and lasts for a while if your lab is the type to reuse antibodies. We're pleased with the results.

Additional Notes

None

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Summary

The Good

Clean, Works Great

The Bad

Super Expensive

The Bottom Line

While this antibody does cost a good deal more than most, it's worth it. Our blots look great and we didn't waste any time having to optimize, which is great. I highly recommend this antibody to anyone blotting for a-tubulin.

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