New England Biolabs Inc
NEBNext Ultra DNA library prep kit for Illumina
E7370S
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One microgram of sheared genomic Bacterial DNA was used for preparing sequencing libraries in Illumina Miseq platform using NEBNext Ultra DNA library prep kit. Prepared libraries have had index and illumina sequencing parts and were size selected for 200 bp which was confirmed upon agilent bioanalyzer analysis. Got 24 ng/microliter libraries at end of reactions which were sufficient to proceed further for sequencing reactions.
Next generation DNA sequencing using Illumina Miseq
Bacterial genomic DNA
One microgram genomic DNA was sheared, end prepped, adaptor ligated using NEBNext ultra DNA library prep kit. Then it was size selected for 200 bp using Agent court AMPure XP beads as indicated in manufacturer's protocol. Adapter ligated DNA fragments (15 microliter) were PCR amplified in 12 cycles using index primer and Universal PCR primer in a total 50 microliter reaction following protocol instructions. Finally PCR products were quantified using qubit ds DNA HS assay kit and analyzed in Agilent bionalyzer.
None. Follow standard instructions from protocols manual.
Using this kit, got consistent 200 bp adaptor ligated libraries with indexes and sequencing parts compatible for Illumina Miseq sequencing.
None
Easy to use protocol. Less hands on time.
Highly recommend for one who wants to prepare bacterial DNA libraries next generation sequencing in Illumina platform
Product Name: NEBNext Ultra DNA Library Prep Kit for IlluminaSupplier Page