eBioscience
Anti-Human CD196 (CCR6) PE
121969
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The aim of this study was to study immune cells in peripheral blood and synovial fluid. Therefore we were interested in the expression of CCR6.
Flow Cytometry
Human peripheral blood
50ul of blood was incubated for 30 minutes at room temperature in the dark with the antibody. After this peripheral blood was lysed using the BD lysing buffer as indicated in the manual. Cells were washed three times with PBS and then resuspended in 300ul of FACS buffer and used for flow cytometry
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Flow cytometry
CCR6 could be clearly identified in CD4+ and CD4- T cells of human peripheral blood. In the figure you see CD3+ T cells that are plotted using the antibody CCR6 and CD4.
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Good staining of CCR6 in human T cells
Using this antibody, CCR6+ CD3+ T cells can be easily identified.