Santa Cruz Biotechnology
Acetylated alpha tubulin
sc-23950
Info: View Product View Product Specs
Browse Similar Products: Acetylated alpha Tubulin Antibodies
The goal of this experiment was to be able to successfully view acetylated tubulin in rat hepatoma cells using immunofluorescence.
Immunofluorescence
H4IIE rat hepatoma cells
1:50 antibody, 1% BSA in PBST (PBS+ 0.1% Tween 20) overnight at 4 degrees C.
1% BSA, 22.52 mg/ml glycine in PBST (PBS+ 0.1% Tween 20) for 30 minutes at room temperature
1:200 Alexa Fluor 555, 1% BSA, in PBST (PBS+ 0.1% Tween 20) for 1 hour at room temperature
NA
Olympus BX63, Cy3 fluorescent filter cube
Acetylated tubulin appears bright under the microscope and easy to detect.
None
Simple protocol. Easily detectable using IF
This acetylated tubulin antibody tags a large percentage of cells and is therefore easy to detect. Recommended antibody dilution is accurate.