EMD Milllipore
Anti-Luciferase, Firefly
Cat. # AB3256-100 µL
Browse Similar Products: Luciferase, Firefly Antibodies
We do work on small molecule inhibitors against microRNA which are involved in breast and liver cancer progression. To find small molecule inhibitors for these microRNAs, we use a dual luciferase system as a screening platform.
Western Blot
HeLa cell lysate
1:3000 dilution, 2hr at room temperature.
Non-fat milk
1hr at room temperature, 1:5000 dilution
NA
ECl from GE, chemi-doc
We validated the firefly and renilla, both expressed properly and we found the specfic band at 66 kd for firefly.
Add the antibody, highest dilution above 1:3000 dilution
Nice antibody
None found
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