Life Technologies
Propidium Iodide
P3566
Info: View Product View Product Specs
Our lab's longstanding goal is to unravel new protein biomarkers in cancer and elucidate their functional role in tumorigenesis. Here, we sought to identify translocation of proteins to the nucleus and for this we tested Propidium iodide (PI) which is a nuclear staining dye that binds to DNA thereby staining the nucleus. Since PI has a fluorescence excitation maximum of 535 nm and the emission maximum of 617 nm, we can use other filters at 488 and 670 to detect other proteins along with nucleus. Here we use PI to detect the nucleus of cancer cells, shown in red in the picture. Staining is intense and bright in color when observed under a confocal microscope.
Immunofluorescence (IFC)
Cancer Cells
Cells were fixed using PFA and then intracellular staining was performed in presence of triton x-100. Cells were then stained for PI at 1:1000 concentration for 15 minutes and then mounted for visualization under a microscope.
Keep ratio at 1:1000
Very good staining of the nuclei, at times we can observe the spindle formation
None
Ease of standardization
Range of fluoroscence
Very good nuclear stain if looking at any 1 other color.
Product Name: Propidium IodideSupplier Page