Santa Cruz Biotechnology
Anti-tyrosine hydroxylase rabbit pAB
sc-14007
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This antibody was used to look at the amount of tyrosine hydroxylase protein in brain homogenates of MPTP-intoxicated mice. Midbrain containing the SN and striatal areas of the brain of mice were isolated, homogenized, and purified. 40 ug total protein was loaded and ran on 4_20% gels. Gels were transferred to PVDF membranes for incubation with Th antibody and HRP-conjugated secondary.
Western Blot
Brain homogenate
1:200 in 5% dry milk/PBST overnight at 4 degrees
5% dry milk/PBST for 45 - 60 minutes at room temp
60 minutes with HRP-conjugated secondary antibody (1:20,000) in 5% dry milk/PBST
NA
Femto Chemiluminescent
Got very good signal even with a relatively short development time of the membrane.
None
Great signal, no non-specific bands, can re-use diluted primary antibody 2 or 3 times
Need to use relatively high concentration.
Works great and consistently on brain homogenate once proper dilution is determined.