Anti-Fibroblasts, Clone TE-7 Does Label Fibroblastic Cells

February 13, 2015

BlueskyReddit
Surgery
University of Nebraska Medical Center
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

EMD Millipore

Product Name:

Anti-Fibroblasts, clone TE - 7

Catalog Number:

CBL271

We wanted to label fibroblastic cells in the gastrocnemius of Peripheral Artery Disease patients to establish the presence of progressive myofibrosis with increasing disease stage. We decided to try this antibody because others reported in the literature to label fibroblasts like pro-collagen and FSP-1 markers did not work or had non-specific labeling. Furthermore, in the literature, TE-7 has been reported to specifically label fibroblastic cells and those of mesenchymal origin, but not immune cells.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Paraffin Embedded Gastrocnemius Sections

Primary Incubation

1:10, 16 hours overnight, 4 degrees Celsius

Blocking Agent

10% Goat Serum

Secondary Incubation

GBI D22-60D Kit, 1 hour, room temperature per manufacturer instructions

Tertiary Incubation

NA

Detection

DAB

Results Summary

This antibody labeled areas of gastrocnemius that were not healthy, where fibroblastic cells would be expected, while appearing less often in healthier areas. We stained a slide section very near with Masson Trichrome Stain and found an association between areas of collagen deposition and labeling, which supports that this antibody is specifically labeling fibroblastic cells.

Additional Notes

If titrating start at the high concentrations.  Must incubate overnight for at least 12 hours or signal will not be very strong. Does not work as well with immunofluorescence. Does not work on Western Blot.

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Summary

The Good

Specifically labels where fibroblastic cells should exist

The Bad

Optimization took a very long time and high concentration is needed which makes it very expensive

The Bottom Line

Works as advertised, expect optimization to be time consuming, and will be very expensive if many histological sections need to be stained

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