Handy Western Blot: Good Results

Overall

Performance

Ease-of-Optimization

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Baylor College of Medicine
HCOA
Postdoc Fellow

Company:

Life Technologies

Product Name:

iBind™ Western Device

Catalog Number:

slf1000

Info: View Product View Product Specs

Browse Similar Products: Blotting Equipment

I was looking for a way to speed up my western blots since I run many gels per week while doing a whole lot of other experiments. I gave this machine (is it really a machine?) a try and was surprised at how neat the membrane turned out. The most amazing thing is that it neither uses any power nor vacuum suction. It just works!The only qualm I have with it is the fact that it can only handle one minigel (or as the rep claimed, multiple strips of membrane equal to the area of one minigel). The primary antibody is also not recyclable. Overall, it's a neat little machine that does the "handsfree" antibody probing process very well.

Experimental Design and Results Summary

Tips

I followed the manufacturer instructions to the letter and got a neat result. Although, I was using an antibody against a high expression protein, I'm not sure how it will do with low expression proteins

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Summary

The Good

Handsfree, neat results

The Bad

Only 1 membrane at a time, involves consumables.

The Bottom Line

The machine does what it was advertised to do.

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