ITGB3 Antibody in A549 Cells Western Blotting

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Kli.Pha.-Tox
Charité - Universitatsmedizin Berlin, CCM
Ph.D.

Company:

Aviva Systems Biology

Product Name:

ITGB3 antibody - N-terminal region (ARP63597_P050)

Catalog Number:

ARP63597_P050

ITGB3 antibody – in A549 cells - human Aveolar Basal Epithelial Cells - Western Blotting

Experimental Design and Results Summary

Applications

Western Blot

Sample

Lysate of A549 cells

Primary Incubation

over night - 4°C (dilution of 1:500 – 1:1000) in TBS-T; 1%BSA; 0,02 Na3

Blocking Agent

5 % low-fat milk solution

Secondary Incubation

1h at room temperature - goat- anti-rabbit – HRP conjugated (DAKO, 1:5000) - in 5 % low-fat milk

Tertiary Incubation

None

Detection

ECL Plus Detection Reagents (Amersham), 5 min incubation

Results Summary

Adequate antibody for WB

Additional Notes

None

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Ok

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