Wako Chemicals USA
Anti-Iba-1 Polyclonal Antibody, Rabbit
019-19741
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For the detection of microglia in mouse and human brain and spinal cord tissues, Iba-1 has been identified as a good marker candidate. Indeed, Iba-1 is specifically expressed in macrophages/microglia and hence using this marker, one can clearly distinguish microglia/macrophages from astrocytes and neurons in the nervous system. The most useful purpose of the Anti-Iba-1 antibody is to differentiate normal quiescent microglia from activated and inflammatory microglia. This is important from the standpoint of neuroinflammation which was my area of research. Specifically, I also wanted to identify how microglia contribute to the growth and development of astrocytes and neurons in the developing embryo and during developmental neuropathogenesis (Antony JM, 2011a,b) .
Immunohistochemistry (IHC), immunocytochemistry (ICC), fluorescence activated cell sorting (FACS)
Frozen and paraffin sections and primary cultures from embryonic and postnatal mouse brain tissue
Iba-1 4°C overnight incubation at 1:1000 in 3% normal goat serum (NGS) and 0.26% bovine serum albumin (BSA) in hepes buffered saline (HBS)
6% NGS, 0.5% BSA in HBS, 1 hour at room temperature.
Alexa488-conjugated goat anti-rabbit (Jackson Immunoresearch, Cat # 111-545-003) at 1:10,000 in 3% NGS and 0.26% BSA in HBS, for 1 hour at room temperature
N/A
Fluorescence (Zeiss Axioplan2 upright microscope with SPOT2 camera)
Iba-1 is exclusively present on microglia in embryonic and postnatal mouse brain tissue. The signal is very strong with no background signal in any other cell type. Poor washing in PBS can lead to low level detection of Iba-1 in other cell types.
Iba-1 as a marker has been well established and has shown consistent results in several experiments. Indeed, Iba-1 transgenic mice are available (Hirasawa et al., 2005).
Anti-Iba-1 polyclonal antibody can be used for several applications (immunocytochemistry and immunohistochemistry, FACS)
None.
Good antibody and probably the best antibody currently available for detection of microglia.