Manual DNA Sequencing

Manual DNA Sequencing DNA sequencing is any process used to map out the sequence of the nucleotides that comprise a strand of DNA. Sequencing is used by researchers in molecular biology or genetics laboratories as well as clinically to make medical treatment decisions and aid in genetic counseling. Manual DNA sequencing was described in 1977 by Maxim and Gilbert’s radiolabelling method, and further refined by Sanger’s chain termination method. The Sanger method, in mass production form, is the technology which produced the first human genome in 2001, ushering in the age of genomics. SInce then, drastic new approaches and automation have been successful in making DNA sequencing faster, easier and more cost effective. However, manual DNA sequencing techniques continue to be used by smaller scale laboratories.
  • GenElute Plasmid Prep Kit From Sigma-Aldrich

    In the modern molecular world almost every biology lab today finds itself working with plasmid DNA in some capacity. One of the simplest tasks required is purifying plasmid DNA from small... read more
  • Sequi-Gen® GT From Bio-Rad

    The Sequi-Gen® GT is a vertical slab gel electrophoresis unit used for high resolution nucleic acid gel electrophoresis, which is crucial in experiments like DNase foot-printing, DNA finger-printing, RNase protection assay... read more